Abstract
By culturing corpora cardiaca-corpora allata complexes from embryos in vitro in the presence of either [ 14C-methyl]methionine or [2- 14C]acetate we have shown that these glands synthesize methyl farnesoate and JH III de novo at stages after dorsal closure. Rates of production of these two substances were found to increase rapidly after dorsal closure and to remain on a similar level until a few days before hatching when a decrease was seen. Methyl farnesoate was by far the predominant product formed under in vitro as well as in vivo conditions until the stage of breaking of the chorion, whereafter JH III became predominant. Our in vitro analyses on JH III degradation in embryos revealed that the egg-case envelope efficiently degraded JH III at all stages investigated, whereas embryo homogenates degraded JH III efficiently only at stages shortly before hatching after the chorion had broken up. The major metabolite of embryos was identified as JH III acid and separate analyses of various tissues indicated that esterases were mainly located in fat body and/or integument and to some extent in gut and haemolymph.
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