Abstract
Polybrene and/or spermidine treatments were used to deliver plasmid DNA into cotton suspension culture obtained from cotyledon-induced callus. The transforming plasmid (pBI221.23) contained the selectablehpt gene for hygromycin resistance and the screenablegus gene. Primary transformant cotton plants were regenerated and analyzed by DNA hybridization and β-glucuronidase assay. The combination polybrene-spermidine treatment greatly enhanced the uptake and expression of DNA and the recovery of nonchimeric germ-line transgenic cotton plants.
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