Abstract
As measured by the breakdown of pulse-labeled RNA or hybridizable mRNA after RNA synthesis was limited with rifampicin, total unstable RNA was at least two-fold more stable in strain AB105. Details of the stabilization have been analyzed for trp operon mRNA. After synthesis was arrested by addition of tryptophan or rifampicin, the functional lifetime of trp (and also of lac) mRNA was normal. However, two pools of trp mRNA molecules were detected by their independent chemical decay. About 70% of the mRNA chains showed a relatively normal chemical lifetime. The other 30%, including both proximal and distal sequences, produced little if any functional proteins, but was 3.5-fold more stable than trp mRNA in the parental strain. Thus, the rate of breakdown of a segment of an mRNA in growing cells is not simply determined by its sequence of nucleotides. Extensive pleiotropic effects accompanied the stabilization of the mRNA. The levels of trp mRNA species were lower than in the parental strain; and total and trp mRNA showed a fraction of relatively short chains.
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