Abstract
BackgroundSplicing factor SRSF3 is an oncogene and overexpressed in various kinds of cancers, however, the function and mechanism involved in colorectal cancer (CRC) remained unclear. The aim of this study was to explore the relationship between SRSF3 and carcinogenesis and progression of CRC.MethodsThe expression of SRSF3 in CRC tissues was detected by immunohistochemistry. The proliferation and invasion rate was analyzed by CCK-8 assay, colony formation assay, transwell invasion assay and xenograft experiment. The expression of selected genes was detected by western blot or real time PCR.ResultsSRSF3 is overexpressed in CRC tissues and its high expression was associated with CRC differentiation, lymph node invasion and AJCC stage. Upregulation of SRSF3 was also associated with shorter overall survival. Knockdown of SRSF3 in CRC cells activated ArhGAP30/Ace-p53 and decreased cell proliferation, migration and survival; while ectopic expression of SRSF3 attenuated ArhGAP30/Ace-p53 and increases cell proliferation, migration and survival. Targeting SRSF3 in xenograft tumors suppressed tumor progression in vivo.ConclusionsTaken together, our data identify SRSF3 as a regulator for ArhGAP30/Ace-p53 in CRC, and highlight potential prognostic and therapeutic significance of SRSF3 in CRC.
Highlights
Splicing factor Ser‐ ine/arginine-rich splicing factor 3 (SRSF3) is an oncogene and overexpressed in various kinds of cancers, the func‐ tion and mechanism involved in colorectal cancer (CRC) remained unclear
SRSF3 upregulation is prevalent in CRC and associates with poor prognosis First, by analyzing the expression of SRSF3 in CRC cases from 20 CRC cases and one tissue microarray including 90 CRC cases, we found significant higher levels of SRSF3 protein in CRC tissues than in normal colorectal tissues (p < 0.001, Fig. 1a, b)
By comparing different clinicopathological features of 90 CRC cases stratified by SRSF3 expression level, we found SRSF3 upregulation significantly associated with poorer differentiation (p = 0.01), more lymph node invasion (p = 0.01), and advanced AJCC stage (p = 0.01, all comparisons by Fisher’s exact test, Table 1)
Summary
Splicing factor SRSF3 is an oncogene and overexpressed in various kinds of cancers, the func‐ tion and mechanism involved in colorectal cancer (CRC) remained unclear. Colorectal cancer (CRC) is one of the most commonly diagnosed cancer in the world, with over one million new cases every year in the world [1]. SR proteins (serine/arginine-rich proteins), functioning as mRNA-binding proteins and alternative splicing factors, have diverse cellular functions including transcription, translation, RNA export, genomic stability, and miRNA processing [2]. Since SR proteins participate in various cellular processes, their aberrant expression may cause various diseases including cancers [3]. SRSF3 (serine/arginine-rich splicing factor 3), known as SRp20, is one of the most famous SR proteins. SRSF3 is a multifunctional protein, and has been found to be involved in various human diseases, including cancers [4].
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
