Abstract
AbstractWe investigated ethidium bromide intercalation into DNA molecule as a model system to test square‐wave voltammetry (SWV) as a suitable method for this purpose We found that 0.13 μg EtBr intercalates into 1 μg dsDNA in average. Further, SWV was utilized for investigation of doxorubicin‐DNA interactions. Intercalated doxorubicin reduced observed dsDNA cytosine and adenine (CA) signal, but also provided new signal called DOXO at −0.35 V. This phenomenon was observed at both single and double stranded DNA standards. We also employed adsorptive transfer stripping technique coupled with SWV for study of doxorubicin‐DNA interactions. Doxorubicin intercalation into dsDNA molecule adsorbed onto working electrode was fast, because we observed considerable changes in CA and DOXO signals after 360 s. Finally, we detected doxorubicin‐DNA adducts formed in doxorubicin treated neuroblastoma cells.
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