Abstract
A novel simple and selective electrochemical procedure is utilized for the determination of Dinoprostone (DIN) in drug substance and pharmaceutical preparation with good recovery and without interference with other excipient. Herein, the electrochemical sensing platform based upon preparing gold nanoparticle sensor on silica modified carbon paste electrode. The surface morphology of the modified electrode was characterized by scanning electron microscope. Different experimental conditions, including electrode composition, effect of pH and scan rate were estimated carefully by cyclic voltammetry to obtain the highest electrochemical response. By using square wave voltammetry a good linear response was obtained in the range of, 2 x 10-5-4 x10-4 mol L-1, and 2 x 10-7-1.6 x 10-4 mol L-1, with low detection limit of 5 x 10-6 mol L-1, and 4.9 x 10-8 mol L-1 by CPE and GNP/SMCPE respectively. The obtained results are in good agreement with those obtained by official method. No electrochemical method was reported before for determination of DIN. The developed method was simple, rapid, economic and challenging to green analytical chemistry.
Highlights
Prostaglandins are essential mediator that are formed in many tissues and adjust many physiological functions, over normal and/ or patho physiological conditions [1,2,3]
The Scanning Electron Microscopy (SEM) image of Carbon Paste Electrodes (CPE) shows that its surface was characterized by a compact surface, isolated and irregularly shaped graphite, while the SEM image of GSMCPE shows that metallic nanoparticles are located at different elevations over the substrate
Preliminary investigation using cyclic voltammetry shows a behavior of irreversible oxidation of DIN at bare CPE, silica gelmodified CPE (SMCPE), and Gold Nanoparticles (GNPs)/SMCPE
Summary
Prostaglandins are essential mediator that are formed in many tissues and adjust many physiological functions, over normal and/ or patho physiological conditions [1,2,3]. Dinoprostone stimulates myometrial contractions in the gravid uterus that are similar to the contractions that occur in the term uterus during labor [7,8]. These contractions are usually sufficient to cause abortion [9]. Few analytical methods were developed and validated for determination of dinoprostone in drug substance, dosage form, human gastric mucosa, and in cultured tumor cells using HPLC with UV, laser induced fluorescence and electrospray ionization tandem mass spectrometric detectors. GC-MS was used for determination of the drug in cultured tumor cells [10]
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