SPL14/17 act downstream of strigolactone signalling to modulate rice root elongation in response to nitrate supply

Abstract

Nitrogen (N) is an essential major nutrient for food crops. Although ammonium (NH4 + ) is the primary N source of rice, nitrate (NO3 - ) can also be absorbed and utilized. Rice responds to NO3 - application by altering its root morphology, such as root elongation. Strigolactones (SLs) are important modulators of root length. However, the roles of SLs and their downstream genes in NO3 - -induced root elongation remain unclear. Here, the levels of total N and SL (4-deoxyorobanchol), and the responses of seminal root (SR) lengths to NH4 + and NO3 - were investigated in rice plants. NO3 - -promoted SR elongation, possibly due to short-term signal perception and long-term nutrient function. Compared with NH4 + condition, higher SL signalling/levels and less D53 protein were recorded in roots of NO3 - -treated rice plants. In contrast to wild-type (WT) plants, SR lengths of d mutants were less responsive to NO3 - condition, and application of rac-GR24 (SL analogue) restored SR length in d10 (SL-biosynthesis mutant) but not in d3,d14 and d53 (SL-responsive mutants), suggesting that higher SL signalling/levels participated in NO3 - -induced root elongation. D53 interacted with SPL17, and inhibited SPL17-mediated transactivation from PIN1b promoter. Mutation of SPL14/17 and PIN1b caused insensitivity of root elongation response to NO3 - and rac-GR24 applications. Therefore, we presented that perception of SLs by D14 led to degradation of D53 via the proteasome system, which released the suppression of SPL14/17-modulated the transcription of PIN1b, and resultedin root elongation under NO3 - supply.