Abstract

Crystals from yeast tRNA Phe were dissolved and compared with tRNA Phe that had not been crystallized. A number of differences were found regarding the interaction with ethidium bromide, the melting point and the circular dichroic signal. These differences were assigned to the presence of spermine in the dissolved crystals indicating a transient stabilization of the conformation of tRNA Phe, probably as a tRNA Phe · spermine complex, after dissolving.

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