Abstract
Background: The purpose of this research was to determine histomorphometric changes in busulfan-induced azoospermia after transplantation of AdSCs in guinea pig. AdSCs were isolated from adipose tissue around the testes of guinea pigs and characterized for mesenchymal properties.Materials and Methods: Guinea pigs were allocated into three groups, including the control group without any intervention. To induce azoospermia, groups 2 and 3 received a dose of 40 mg/kg of busulfan with 21 days interval. Group 3 received 1×106 AdSCs in their seminiferous tubules of left testes, 35 days following last busulfan injection, while right testis in the group was considered for comparison as controls. Sixty days following transplantation of cell, histomorphometric and histopathologic changes of the experiments were assessed.Results: After AdSCs’ transplantation, normal spermatogenesis appearance was noticed compared to busulfan-induced azoospermia and AdSCs recovered spermatogenesis, and our findings can be added to the literature in treating azoospermic infertilities.Conclusion: The transplanted AdSCs could induce production of germinal cells using testicular seminiferous tubules and were an effective source in treating azoospermia.[GMJ.2018;7:e1000]
Highlights
Infertility is still a crucial social issue in many nations, while male infertility was demonstrated to be responsible for half of GMJCell Transplantation in Azoospermia is classified into two types of non-obstructive or obstructive azoospermia, each with highly variable treatments and etiologies [4]
Characterization of Adipose Tissue-Derived Stem Cells (AdSCs) by Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) AdSCs were found positive for CD90 expressions as Mesenchymal stem cells (MSCs) markers and negative for CD34 as a hematopoietic cell marker (Figure-2)
Adipogenic induction was verified by observation of lipid droplets, and in osteogenic medium, calcium deposits were visualized by confirming osteogenic differentiation of AdSCs (Figure-3)
Summary
Infertility is still a crucial social issue in many nations, while male infertility was demonstrated to be responsible for half of GMJCell Transplantation in Azoospermia is classified into two types of non-obstructive or obstructive azoospermia, each with highly variable treatments and etiologies [4]. Mesenchymal stem cells (MSCs) possess similar features, including favorable proliferative capability, self-renewal, and differentiation potential [15] These cells were separated from bone marrow [16], menstrual blood [17], adipose tissue [18], and dental pulp [19]. The adipose tissue MSCs (AdSCs) are ubiquitous and provided in large numbers with slight donor site injuries or discomforting of patient, making them an appropriate research tool and therapy [24] This investigation assesses histomorphometric and histological changes in guinea pig following AdSCs transplantation in busulfan-induced azoospermia. The purpose of this research was to determine histomorphometric changes in busulfan-induced azoospermia after transplantation of Adipose Tissue-Derived Stem Cells (AdSCs) in guinea pig. Conclusion: The transplanted AdSCs could induce production of germinal cells using testicular seminiferous tubules and were an effective source in treating azoospermia. [GMJ.2018;7:e1000] DOI:10.22086/gmj.v0i0.1000
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