Abstract
The sperm surface glycocalyx has long been known to serve an instrumental role in sperm capacitation and zona pellucida penetration; however, utilization of this aspect of sperm for assisted reproduction or diagnostic purposes has been lacking. We describe novel orthogonal chemistry methodologies to characterize the network of sperm sialic acids; this is with the purpose of developing a quantitative measurement of sperm fertility that can be used as a novel sperm separation tool.
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