Abstract
Abstract Sperm motility performance is strongly linked to the reproductive success of a fish. Since fish’s sperm cells are extruded in the aquatic medium where fish live and reproduce, these tiny cells have to counter all the problems of environmental contamination or pesticide pollution. To overview the perspective, an experiment was accomplished to elucidate the effects of different sub-lethal concentrations [0.00ppm, 0% of median lethal concentration (LC50); 0.47ppm, 10% of LC50; and 0.94ppm, 20% of LC50] of quinalphos 25EC (QP) on sperm motility parameters such as - motility (MOT %), curvilinear velocity (VCL), straight line velocity (VSL), and linearity (LIN %) and morphological changes of silver barb (Barbonymus gonionotus) sperm. After partial dilution in the extender (Alsever’s solution), the fish sperm were exposed to QP for 2 (hour, h) or make the final dilution step during video recording on the microscope stage. The results of the study showed that compared with fresh sperm, incubating for 2 h at 4 °C reduced sperm motility. Incubation with QP for 2 h had a significant (P
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