Abstract
This study focuses on the behavior of a new fluorescent marker for labeling individual biomolecules and staining cell organelles developed on a meso-substituted BODIPY platform. Boron(III) complex with meso-4-methoxycarbonylpropylsubstituted 3,3’,5,5’-tetramethyl-2,2′-dipyrromethene has been synthesized and identified via visible, UV-, NMR- and MS-spectra X-ray. The behavior of fluorophore in solutions has been studied with various experimental techniques. It has been found that luminophore exhibits a high quantum yield (almost ~100–75%) in the blue-green region (513–520 nm) and has high photostability. In addition, biological analysis indicates that the fluorophore exhibits a tendency to effectively penetrate into cell membranes. On the other hand, the proposed BODIPY can be used to study the significant differences among a large number of pathogens of mycotic infections, as well as to visualize structural changes in the plasma membrane, which is necessary for the clearance of mammalian cells undergoing apoptotic cell death.
Highlights
Nowadays, microscopic fungi are considered to be causing a significant increase in serious diseases in humans, animals and plants
The possible use of [BF2 L] as a fluorescent marker on pathogenic microorganisms has been analyzed by staining several strains of microscopic fungi C. albicans and F
It would seem that our findings allow us to use the synthesized [BF2 L] phosphor as a marker in assessing the membrane structures of fungal cells, which may help in the study of the existing significant differences among a large number of mycotic infections pathogens
Summary
Microscopic fungi are considered to be causing a significant increase in serious diseases in humans, animals and plants. An actual task is to search for new BODIPY biomarkers with a high quantum yield, low sensitivity of fluorescence to the properties of biological media and efficient transport across the cell membrane due to the unimpeded immersion of the molecule into the lipid bilayer. The development of such a group of phosphors will allow us to identify new structures necessary for establishing a mechanism for regulating the molecular functioning of cells and a detailed study of cell morphology.
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