Abstract
The coloured complex formed on reaction of quercetin (3,3′, 4′, 5,7-pentahydroxyflavone) with vanadyl sulphate is used as the basis for a sensitive and reproducible spectrophotometric method for estimation of quercetin. The 1∶1 complex exhibits maximum absorption at 425 nm in aqueous ethanol (80% v/v) adjusted to pH 3.3. Beer's law is followed over the quercetin concentration range of 0.3–20 μg/ml. The molar absorptivity is 1.4×104 l·mole−1·cm−1. This method can be used for the determination of quercetin isolated from biological sources. In the metal complex, the 4-carbonyl and 3-hydroxy groups of the quercetin are involved.
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