Abstract
A simple, rapid, sensitive and accurate method for the determination of aciclovir, cefepime HCI, etamsylate and metoclopramide HCI in pure form and in pharmaceutical formulations is developed.The method is based on the fomabon of tris(o-phenanthroline) iron(ll) complex (Ferroin) upon the reaction of the ated drugs wrth iron(lll )-o- phenanthroline mixture. The ferroin complex is colorimetncally measured at λmax 510 nm against a reagent blank. 0ptimization of the experimental conditions is described. Beer's law is obeyed in the concentration range from 0.25–30 µg ml−1 with molar absorpitivities (ε) ranging from 4.796 x 103–9.51 2 x 104 L.mol−1.cm−1 and Sandell sensitivities (S) of 2.129 x 10−3–34.5 x 10−3 µg cm−2. The developed method is applied successfully for the determination of the cited drugs in pure forms and in the corresponding pharmaceutical formulations without any interferences from common excipients.
Highlights
- 34.5 x 10" pg cm- he developed method is applied successfully for the determination of the cited drugs in pure forms and in the corresponding pharmaceutical formulations wtthout any interferences from common excipients
Elazazy et al.: Cefepime hydrochloride is a fourth generation cephalosporin that's widely used as anti-bacterial and administred via injection"'.lt has been determined by several procedures e.g. spectrophotometry~g~,diffusreflectance IRB X-ray diff~acbon''~) and HPLC'")
The aim of the present work is to apply 1.10-phenanthroline - ~ e re~age' nt to react with the studied drugs yielding a colored ferroin complex and presenting a simple and rapid assay procedure for the studied drugs in pure form and in pharmaceutical forrnulations.This work describes a spectrophotometnc method that can be used in laboratories where modem and expensive apparatus such as that required for GLC or HPLC is not available
Summary
- 34.5 x 10" pg cm- he developed method is applied successfully for the determination of the cited drugs in pure forms and in the corresponding pharmaceutical formulations wtthout any interferences from common excipients. Elazazy et al.: Cefepime hydrochloride is a fourth generation cephalosporin that's widely used as anti-bacterial and administred via injection"'.lt has been determined by several procedures e.g. spectrophotometry~g~,diffusreflectance IRB X-ray diff~acbon''~) and HPLC'"). The aim of the present work is to apply 1.10-phenanthroline - ~ e re~age' nt to react with the studied drugs yielding a colored ferroin complex and presenting a simple and rapid assay procedure for the studied drugs in pure form and in pharmaceutical forrnulations.This work describes a spectrophotometnc method that can be used in laboratories where modem and expensive apparatus such as that required for GLC or HPLC is not available. -1s and Reagents : All chemicals used were of analybcal grade and all solutions were freshly prepared in doubly distilled water
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