Abstract

The new Leica TCS SP5 spectral confocal and multiphoton microscope system is based on the widely used SP® spectral detection module. This module allows selection of any spectral emission band in the visible range. Five spectral bands can be selected simultaneously. As an extension, two of these detectors may operate as a sensor for fluorescence lifetime imaging microscopy (FLIM). This SP-FLIM combination opens up a completely new dimension in wavelength-dependent lifetime imaging.

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