Abstract
Pseudomonas aeruginosa (Pae) is an opportunistic pathogen showing a high intrinsic resistance to a wide variety of antibiotics. It causes nosocomial infections that are particularly detrimental to immunocompromised individuals and to patients suffering from cystic fibrosis. We provide a snapshot on regulatory RNAs of Pae that impact on metabolism, pathogenicity and antibiotic susceptibility. Different experimental approaches such as in silico predictions, co-purification with the RNA chaperone Hfq as well as high-throughput RNA sequencing identified several hundreds of regulatory RNA candidates in Pae. Notwithstanding, using in vitro and in vivo assays, the function of only a few has been revealed. Here, we focus on well-characterized small base-pairing RNAs, regulating specific target genes as well as on larger protein-binding RNAs that sequester and thereby modulate the activity of translational repressors. As the latter impact large gene networks governing metabolism, acute or chronic infections, these protein-binding RNAs in conjunction with their cognate proteins are regarded as global post-transcriptional regulators.
Highlights
High-throughput RNA sequencing (RNAseq) has revolutionized the discovery of regulatory RNAs in bacteria [1,2,3,4,5,6,7,8,9,10,11,12,13]
Once the trigger is lost, the regulatory RNA is often co-degraded with the target mRNA [14]
Hfq In canGram-negative act as an RNA matchmaker, aiding in Pseudomonas aeruginosa (Pae) base-pairing between small basepairing RNAs (sRNAs) and their mRNAs, especially cognate sRNA/mRNA
Summary
High-throughput RNA sequencing (RNAseq) has revolutionized the discovery of regulatory RNAs in bacteria [1,2,3,4,5,6,7,8,9,10,11,12,13]. Regulatory RNAs are diverse and can act at all levels of gene expression, by (i) controlling DNA maintenance and silencing [15,16,17], (ii) activating or repressing transcription [18,19,20,21,22], (iii) affecting translation and/or stability of target mRNAs [2,13,23,24,25], (iv) modulating the function of proteins [18,19,21,26,27,28,29], or (v) by sequestering other sRNAs [30,31,32]. We focus on base-pairing sRNAs and protein-binding RNAs in the opportunistic human pathogen Pae
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