Abstract
Plasmonic nanoparticles are appealing for biomolecule and drug delivery due to their unique photophysical properties that provide spatiotemporally controlled release capabilities. Emerging techniques using light to control cellular functions, such as in optogenetics, motivates the design of novel methods to achieve mechanical manipulation of the cell. Here we demonstrate the release of a recombinant green fluorescent protein fused with a poly‐histidine tag to facilitate attachment to ~ 40 nm hollow gold nanoshells by nickel‐nitrilotriacetic acid affinity as well as a C‐terminal targeting peptide to promote cellular uptake of the nanoparticle‐protein composite. Laser release of protein was characterized by fluorescence for both particle and cellular studies, which was found to be power and time dependent. The construct presented may serve as a platform for further studies of protein delivery using nanoparticles.Grant Funding Source: This work was supported by National Institutes of Health (NIH) grant R01 EB012637.
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