Abstract
Knowledge of the spatial distribution of lipids in the intraocular lens is important for understanding the physiology and biochemistry of this unique tissue and for gaining a better insight into the mechanisms underlying diseases of the lens. Following our previous study showing the spatial distribution of sphingolipids in the porcine lens, the current study used ultra performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOFMS) to provide the whole lipidome of porcine lens and these studies were supplemented by matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) of the lens using ultra-high resolution Fourier transform ion cyclotron resonance mass spectrometry (FTICR MS) to determine the spatial distribution of glycerophospholipids. Altogether 172 lipid species were identified with high confidence and their concentration was determined. Sphingomyelins, phosphatidylcholines, and phosphatidylethanolamines were the most abundant lipid classes. We then determined the spatial and concentration-dependent distributions of 20 phosphatidylcholines, 6 phosphatidylethanolamines, and 4 phosphatidic acids. Based on the planar molecular images of the lipids, we report the organization of fiber cell membranes within the ocular lens and suggest roles for these lipids in normal and diseased lenses.
Highlights
The ocular lens is a unique epithelial tissue responsible for finetuning images projected onto the retina
We elucidated the spatial distribution of sphingolipids in the porcine lens using Matrix-assisted laser desorption ionization (MALDI) Mass spectrometry imaging (MSI) and provided detailed information about compositional changes in the clear lens nucleus and cortex [9]
We provide the whole lipidome of clear porcine lens and determine the spatial distribution of 30 glycerophospholipids (GPs) using a combination of ultra performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOFMS) and MALDI imaging coupled to Fourier transform ion-cyclotron resonance mass spectrometry (FTICR MS)
Summary
The ocular lens is a unique epithelial tissue responsible for finetuning images projected onto the retina. The changes in the distribution of spatial phospholipids were assessed based on the compositional average from relatively large sections of the lens, and the spatial resolution of the experiment was low and no detailed differences in lipid concentrations were observed. The in situ spatial analysis of the phospholipids of the lens by direct MALDI-TOF MS analysis was conducted by averaging mass spectra acquired from three or four spots next to each other from the cortex and the nucleus [7].
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