Abstract
BackgroundGlioma is the most frequent malignant primary brain tumor in adults.ObjectiveTo explore the role of sperm-associated antigen 5 (SPAG5) in glioma.MethodsThe association between SPAG5 expression and clinical features was investigated based on The Cancer Genome Atlas (TCGA) datasets. The function of SPAG5 in glioma was analyzed using U87 and U251 cells. Knockdown glioma cells were constructed by shRNA interference. qRT-PCR and Western blotting were used to measure the expression of SPAG5 and Cadherin 2 (CDH2). Cell proliferation and apoptosis were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, caspase 3/7 assay, and high-content screening (HCS) proliferation analysis and colony formation assay. Transwell assays and wound-healing assays were used to investigate cell migration and invasion.ResultsThe increased expression of SPAG5 was correlated with poor outcomes in glioma patients. Knocking down SPAG5 could inhibit the proliferation and colony formation and promoted the apoptosis of glioma cells. Knocking down SPAG5 could also inhibit cell migration and invasion and the expression of CDH2. Overexpression of CDH2 with SPAG5 depletion could restore the proliferation and inhibit the apoptosis of glioma cells, which also promoted cell migration and invasion.ConclusionsSPAG5 is a promising prognostic factor and potential therapeutic target for clinical intervention in glioma.
Highlights
Glioma is a neuroectodermal tumor arising from glial or precursor cells [1], which represents one of the most frequent malignant neoplasm in the central nervous system [2]
Our results provided the evidence that downregulation of SPAG5 represses glioma cell proliferation and attenuates glioma cell migration and invasion in vitro
According to the median of the SPAG5 mRNA expression, the 667 glioma specimens were further divided into low SPAG5 expression group (n = 334) and high SPAG5 expression group (n = 333)
Summary
Glioma is a neuroectodermal tumor arising from glial or precursor cells [1], which represents one of the most frequent malignant neoplasm in the central nervous system [2]. Studies demonstrated that glioma accounts for about 75% of primary malignant brain tumors in adults [3, 4]. Most of the glioma patients with high grade succumb to this. Sperm-associated antigen 5 (SPAG5, called astrin and hMAP126), which maps to Ch17q11.2 and codes for a mitotic spindle-associated protein [8], plays a key role in the regulatory network of mitosis by forming a molecular switch with a mass of protein partners [9]. Glioma is the most frequent malignant primary brain tumor in adults
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