Soy germ for the prevention of prostate carcinogenesis in TRAMP mice (824.9)

Abstract

Prostate cancer (PCa) is the most commonly diagnosed cancer and second leading cause of cancer deaths in United States men. Soy germ has higher levels of daidzein and glycitein, and lower amounts of genistein compared to typical soy products. Genistein has been well studied, but less is known about daidzein and its microbially‐produced metabolite, (‐) equol, in PCa development. The objective of this research is to investigate the effect of feeding 2% soy germ, daidzein, or (‐) equol on the progression of PCa in the transgenic adenocarcinoma of the mouse prostate (TRAMP) model. 3‐week old male C57BL/6 X FVB TRAMP mice were weaned from our breeding colony and immediately acclimated to a custom AIN‐93G control diet for one week. At 4 weeks of age, mice (n=30 per diet group) were randomized to one of four study diets, AIN‐93G control, AIN‐93G + 2% soy germ, AIN‐93G + 92 ppm daidzein, or AIN‐93G + 88 ppm (‐) equol until 18 weeks of age. Levels of daidzein and (‐) equol were matched to daidzein equivalents found in a 2% soy germ diet. Preliminary data shows no differences in food intake or body weight between the groups. Further analyses to be completed include prostate pathology scores, serum inflammatory markers, and epigenetic changes in prostate tissue. At the conclusion of this study, we will determine if a previously observed protective effect of soy germ is associated with the daidzein component or the daidzein metabolite, (‐) equol.Grant Funding Source: Supported by NIH P50 AT006268