Southern Blight of Tall Fescue and Bluegrass Caused by Sclerotium rolfsii in Italy

Abstract

Tall fescue (Festuca arundinacea Schreb.) and Kentucky bluegrass (Poa pratensis L.) are the main turfgrass species cultivated in Sicily (southern Italy) for ready lawn (sod) to ornamental purposes. In July 2004 and May 2005, a widespread disease was noticed in two turf nurseries on the eastern side of Sicily on a ready lawn mixture of F. arundinacea cv. Safari (94%) + P. pratensis cv. Cabaret (6%). Numerous yellow, circular- and crescent-shaped patches as much as 30 to 40 cm in diameter were observed. The turf usually died around the perimeter of the patch, but the grass remained green in the center of the ring with a tuft of green grass in the center (frog eye). Affected turf was initially reddish brown and turned brown as it died. Small, round and off-white or tan seed-like structures were dispersed on mycelial strands at the outer edge of the ring in the mat at the base of grasses. The pathogen was identified as Sclerotium rolfsii Sacc. The fungus was isolated directly as aerial mycelium or sclerotia or following surface disinfection (2 min in 0.5% NaOCl) and plating diseased tissues on potato dextrose agar (PDA). Sclerotia were observed in vitro in 7-day-old cultures. Pathogenicity was tested by inoculating two com-mercial ready lawn strips (80 × 100 cm) of two healthy turfgrass species each with three isolates of the fungus. Thirty sclerotia were placed at the base of stems. Noninoculated ready lawn strips served as control. All plants were covered with plastic bags, exposed to diffused daylight for 5 days, and then maintained in a growth chamber at 25 to 28°C under fluorescent light. Disease symptoms and southern blight signs like the ones observed in the field occurred 2 weeks after inoculation. S. rolfsii was reisolated from affected tissues. Symptoms were not detected on any of the non-inoculated ready lawn strips. The disease was serious enough that chemical treatments were required for its control. Southern blight was previously detected on bermudagrass and other cool-season turfgrass genera (1).To our knowledge, this is the first report of southern blight on tall fescue and bluegrass in Italy. Reference: (1) R. W. Smiley. Common Names of Plant Diseases. Diseases of Turfgrasses. Online publication. The American Phytopathological Society, St. Paul, MN.