Abstract
Chitosan is soluble in diluted acid solutions and can easily form films by casting. However, residual acid neutralization should be performed for biomedical applications what may compromise physical and mechanical properties of the films. Thus, plasticizers can be added to improve these properties. The aim of this study was to characterize morphological, barrier and mechanical properties, besides evaluate the in vitro cytotoxicity of sorbitol-plasticized and NaOH-Na2CO3 neutralized chitosan membranes for skin substitute application. Scanning electron microscopy, X-ray diffraction, water vapor permeability and mechanical tests were carried out to characterize the obtained membranes. Moreover, Vero cells were used for in vitro cytotoxicity evaluation. In this paper, we report a non-cytotoxic sorbitol-plasticized chitosan membrane with desirable properties for skin substitution, such as flexibility, water vapor permeability and high percentage of elongation.
Highlights
The loss or failure of an organ or tissue is one of the most frequent, devastating, and costly problems in human health care
Chitosan membranes were prepared by solvent evaporation technique. 1.0% (w/w) Acetic Acid (Synth/Brazil) solution was used as solvent. 1.5g of High Molecular Weight Chitosan (Sigma-Aldrich/USA) was dissolved in 100 ml of solvent
The Index of crystallinity (Ic) may be used to compare the content of crystalline fraction of a number of samples. It differs from the degree of crystallinity that indicates the percentage of the crystalline fraction in the polymer[15]
Summary
The loss or failure of an organ or tissue is one of the most frequent, devastating, and costly problems in human health care. Tissue engineering appears to be the new frontier of medicine dedicated to regenerative and reconstructive procedures in humans by developing powerful new therapies to treat structural and functional disorders impossible to treat successfully with the current approaches of interventional medicine[1]. Most of them were designed for permanent use, some of them as temporary substitutes. They contain cells of different origin (autologous, allogeneic or xenogeneic) and biodegradable materials (naturally occurring or synthetic polymers) as scaffolds for cell attachment and facilitated handling. The challenge that remains is the generation of a complex dermis-epidermal substitute that can be securely and conveniently transplanted with minimal scarring in one single surgical intervention[2]
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