Abstract

Escherichia coliare the most common cause of diarrhea in calves. Diarrhea in calvesremains one of the most important problems faced by livestock, causing greateconomic losses. Some strains ofE. coli characterized by the presence of specificvirulence factors including haemolysin production, resistance to bactericidal effectsof serum and Congo red binding activity. In this studyfecal samples were collectedfrom 115 diarrheic calves aged from 3 days to one year and from different localities in Egypt along the period from February to August 2015.The prevalence of E. coli in diarrheic calves was 72.2%. 39.8% of isolated E. coli were haemolytic to sheep blood agar, 68.7% were serum resistant, 100% showed Congo red binding activity.

Highlights

  • Escherichia coli (E. coli) are a normal inhabitant of the gastrointestinal tract of worm blooded animals(Wray and Morris, 1985; Magalhães et al, 2016)

  • Several studies confirmed that, binding of Congo red dye has been associated with pathogenicity of the E. coli (Berkhoff and Vinal, 1986; Vinal, 1988; Gjessing and Berkhoff, 1989). Some strains ofE. coli characterized by resistance to bactericidal effects of serum (Fecteau et al, 2001)

  • Isolation and Biochemical Identification: Out of 115 collected fecal samples from diarrheic calves, 83 E. coli were recovered with a prevalence of 72.2%

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Summary

Introduction

Escherichia coli (E. coli) are a normal inhabitant of the gastrointestinal tract of worm blooded animals(Wray and Morris, 1985; Magalhães et al, 2016). Aim of the work: The present work aimed to identify E. coli causing diarrhea in calves and detect some virulence factors of the isolated E. coli strains. E. coli isolates were tested for their growth status on Congo red medium after incubation for 24 hours at 35 C, left at room temperature for additional 2 days (not to exceed 4 days).

Results
Conclusion

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