Abstract
[5‐3H]Uridine incorporation into ribosomes and other postribosomal ribonucleoprotein particles has been determined in mouse plasmocytoma MOPC406 cytoplasm after cell wall disruption. It has been shown that a large part of the radioactivity after 40 to 90‐min incubation is localized in the postribosomal zone of the gradient. The question arises whether these particles are the result of nuclear leakage during cell disintegration or whether they are native to the cytoplasm. To clarify this we have compared the following characteristics of cytoplasmic and nuclear ribonucleoprotein particles: (a) sensitivity to sodium deoxycholate; (b) sedimentation patterns in sucrose gradients; (c) buoyant density values in CsCl gradient; (d) dynamics of precursors incorporation into cytoplasmic ribonucleoprotein particles RNA.All these results have been considered as supporting the cytoplasmic origin of cytoplasmic postribosomal ribonucleoprotein particles.
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