Abstract
Highly polymerized herring testis DNA of the random nucleotide sequence was used as a model of natural substrate to study some features of hydroly sis of the hybrid B-Z form with Serratia marcescens nuclease. The hybrid B-Z form was formed upon addition of 1.15 M MgSO 4 and 0.421 mM Co(NH 3)6Cl 3. The DNA transition from the right handed B-form t o the hybrid B-Z-form caused a decrease in Vmax of DNA cleavage with the nuclease. The diminishing Vmax was consistent with diminishing values of Km and Kcat. The binding of Mg 2+ or Co(NH 3)6 3+ to highly polymerized DNA caused correspondingly about 80-or 7-fold decrease in Km and more than 1600 or 600 decrease in Kcat compared with that of Mg-DNA complex of B-form.
Highlights
Current research findings leads to the assumption that within the same molecule of natural DNA radicallyPurine/pyrimidine sequences, poly d(G-C), poly different geometries coexist and the hybrid B-Z-form of d(A-T) and poly d(A-C) x poly d(G-T), were found to DNA containing left-handed fragments separated by a fold into a left-handed helix, Z-type conformation, B-Z junction from right-handed regions really exists in under reduction of water activity
Circular Dichroism Spectrometry (CD) spectra of the DNA in the absence and in the presence of 1.15 M MgSO4 are shown in Fig. 1A
CD spectrum of the DNA in the presence of 1.15 M MgSO4 had both decreased towards the control line peak around 275 nm and a trough at 243 nm
Summary
Purine/pyrimidine sequences, poly d(G-C), poly different geometries coexist and the hybrid B-Z-form of d(A-T) and poly d(A-C) x poly d(G-T), were found to DNA containing left-handed fragments separated by a fold into a left-handed helix, Z-type conformation, B-Z junction from right-handed regions really exists in under reduction of water activity The B- found across the human genome in neighboring to Z- helix transition has been mainly studied on short poly- regions of protein-encoding genes (Champ et al, or oligodeoxynucleotide fragments, with strictly alternating 2004). Z-DNA regions are often met near purine/pyrimidine sequences. The transcription, as was deoxyoligonucleotides containing both poly d(G-C) reported (Lancillotti et al, 1987), can induce the Z-
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