Abstract
Polyacrylamide gel electrophoresis in discontinuous buffer systems often gives very narrow zones. This technique involves some disadvantages, however, which are discussed. Although our experience so far is rather limited, it would indicate that the resolving power is independent of whether a continuous or discontinuous buffer system is used, provided that the starting zone is sharpened to the same width in the two systems. A zone sharpening can easily be achieved by giving the sample a lower conductivity than the buffer.
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