Abstract
Canna indica Linn. (Cannaceae) is used both as medicine and food. Traditionally, various parts of C. indica are exploited to treat blood pressure, dropsy, fever, inflammatory diseases etc. However, to date there is no reliable micropropagation protocol for C. indica. We present here a regeneration technique C. indica with banana micropropagation medium (BM). BM supplemented with 3% sucrose, 0.7% agar, and 0.17% NH4NO3 and different plant growth regulators like BAP (2 mg∙L−1) and NAA (0.5 mg∙L−1) was found to be effective in inducing callus. BM with BAP (2 mg∙L−1) was ideal for somatic embryogenesis and plantlet regeneration. After a period of 3 months, regenerated plantlets were successfully transferred to the field conditions. Appearance of somaclonal variation among the regenerated plants is a common problem which was assessed by DNA fingerprinting. To detect genetic fidelity in C. indica, RAPD and ISSR markers were employed. Ten RAPD primers produced 60 amplicons, while 7 ISSR primers generated 45 bands in both in vitro plantlets and mother plants. RAPD and ISSR analyses showed no evidence of polymorphism between parent plants and the regenerated plants as all the amplified products were found to be monomorphic.
Highlights
Canna, the solitary genus of the family Cannaceae, is a well-known ornamental plant with beautiful flowers
Callus-like structures of the in vitro maintained leaves were observed after 15–20 days of culture (Figure 1)
banana micropropagation medium (BM) supplemented with 2 mg·L−1 benzyl amino purine (BAP) and 0.5 mg·L−1 napthaleneacetic acid (NAA) assisted profuse callusing
Summary
The solitary genus of the family Cannaceae, is a well-known ornamental plant with beautiful flowers. The plant is cultivated extensively throughout the world for its decorative and widely-varying flower colors [1]. Species of Canna are edible [2] and have medicinal properties [3,4,5,6]. The common practice for Canna propagation is asexual, mainly through multiplication of rhizomes. This poses the problem of genetic stagnancy so genetic variation is limited. Varietal improvement of Canna lies in genetic manipulation and protoplast fusion [7]. In both the cases a preliminary requirement is a robust micropropagation protocol.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
