SOMATIC EMBRYOGENESIS AND ADVENTITIOUS SHOOT REGENERATION FROM LEAF EXPLANTS AND STEM NODES OF

Abstract

The present study was carried out in Plant Tissue Culture Laboratory Department of Horticulture , College of Agriculture & Forestry , Mosul University to propagate lilium plants Lilium longiflorium cv. Aster lily a white flowers in vitro by using nodes from plants growing in field in 5 May after flowering and cultured in MS medium supplemented with different concentrations of Kinetin or BA and rooting the shoots produced in vitro , as well as to explain the effect of sucrose on bulblet produced on rooted shoots , also medium or basal parts of leaves were cultured on MS medium supplemented with different concentrations of BA interacted with different concentrations of 2,4-D or on MS medium supplemented with BA and NAA , nodes taken from plants grown in field also cultured in 10 July before plant dormancy on MS medium supplemented with different concentrations of 2,4-D . Data refers that highest number of shoots 8.1 shoot/explant obtained from nodes cultured on MS medium supplemented with 0.5 mg/l BA after eight weeks from planting date , 100% rooting and highest number of roots 11.8 root/explant was obtained from cultured shoots produced in vitro on MS medium supplemented with 0.5 mg/l IBA , highest weight and volume 0.997gm , 0.43 cm3 for bulblet were obtained from cultured rooted shoots on MS medium supplemented with 120 gm/l sucrose , callus produced from culturing nodes three time on MS medium with 0.2 mg/l 2,4-D produced 50% shoots with average 5 shoot /explant after cultured on MS medium without growth regulators and stages of somatic embryogenesis were observed (globular, heart, torpedo) on callus, shoots initiated directly on basal leaf parts cultured on medium supplemented with 0.1 mg/l 2,4-D , otherwise callus initiated on basal leaf parts cultured on medium supplemented with 0.5 mg/l BA and 0.1mg/l NAA and when recultured these callus somatic embryogenesis were observed on it , all plantlets produced in vitro acclimated in laboratory and transferred to plastic house and then to field with 100% survival.