Abstract
The kappa immunoglobulin (Ig) genes from rat kidney and from rat myeloma cells were cloned and analyzed. In kidney DNA one κ species is observed by Southern blotting and cloning in phage vectors; this gene most likely represents the embryonic configuration. In the IR52 myeloma DNA two κ species are observed: one in the same configuration seen in kidney and one which has undergone a rearrangement. This somatic rearrangement has brought the expressed V region to within 2.7 kb 5′ of the κ coding region; the rearrangement site is within the κ cluster which we have mapped. The rat somatic Ig rearrangement, therefore, closely resembles that seen in mouse Ig genes. In the rat embryonic fragment two κ segments were mapped at 2 and 4.3 kb 5′ from the κ coding region. Therefore, the rat κ cluster extends over about 2.3 kb, a region much longer than the 1.4 kb of the mouse and human jk clusters. In the region between κ and the expressed jk of IR52 myeloma DNA, an XbaI site present in the embryonic kappa gene has been lost. A somatic mutation has therefore occurred in the intervening sequence DNA approx. 0.7 kb 3′ from the V/J recombination site. Southern blots of rat kidney DNA hybridized with different rat vk probes showed non-overlapping sets of bands which correspond to different subgroups, each composed of 8–10 closely related κ genes.
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