Abstract
Experiments were made to investigate the technology and conditions for producing chicken manure regenerative feed containing poly-γ-glutamic acid (γ-PGA) under solid-state fermentations (SSF) fromBacillussubtilisCCTCC202048. The maximum γ-PGA production (5.4%) was obtained in the mixed substrates of chicken manure, soybean cake and wheat bran (1:1:0.2 w/w) supplemented with 0.5% glutamic acid and 0.5% citric acid, with initial moisture content 65% and initial pH 8.5 in 250 ml flasks, inoculation at mid-log phase with a 4% inoculum level and cultivation for 48 h at 37°C. Scale-up experiments on the scale of 130 kg also showed that the yield of γ-PGA reached 4.2% and fermentation increased protein content of the solid substrates from 17.5% to 32.8%. These would lay a foundation for lessening the pollution of chicken manure, increasing chicken manure feed efficiency and exploring a late-model feed additive.
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