Abstract

“Water window” soft X-ray has some advantages in the microimaging of biological specimens. Soft X-ray holography can give us three-dimensional images of biological cells in their natural condition. Quasi-monochromatic soft X-ray from the pdymonochromatic synchrotron radiation is obtained by a zone plate-pinhole linear monochromator at National Synchrotron Radiation Laboratory (NSRI,) at Hcfei. The pinhole is used to increase the temporal coherence of the soft X-ray. and also to increase the spatial coherence of it. An in-line holographic experiment has been finished with soft X-ray of main wavelength 4.5 nm and pinhole of diameter 0.03 mm. The magnified hologram is recowtructed with He-Ne laser. A smaller pinhole is wanted to be used to improve the cuherence of the soft X-ray so as to improve the quality of the hologram.

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