Abstract
We investigated the chemical toxic agent sodium cyanate (NaOCN) on the large conductance calcium-activated potassium channels (BK Ca) on hippocampal neuron-derived H19-7 cells. The whole-cell and cell-attach configuration of patch-clamp technique were applied to investigate the BK Ca currents in H19-7 cells in the presence of NaOCN (0.3 mM). NaOCN activated BK Ca channels on H19-7 cells. The single-channel conductance of BK Ca channels was 138 ± 7 pS. The presence of NaOCN (0.3 mM) caused an obvious increase in open probability of BK Ca channels. NaOCN did not exert effect on the slope of the activation curve and stimulated the activity of BK Ca channels in a voltage-dependent fashion in H19-7 cells. The presence of paxilline or EGTA significantly reduced the BK Ca amplitude, in comparison with the presence of NaOCN. These findings suggest that during NaOCN exposure, the activation of BK Ca channels in neurons could be one of the ionic mechanisms underlying the decreased neuronal excitability and neurological disorders.
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