Abstract

A description is given of a simple and rapid spectrophotometric technique for determining the ratio oxyhaemoglobin/haemoglobin in a small quantity of blood. In a comparison of a series of sixty determinations with the Van Slyke method, which is used as a reference method, there has been excellent agreement between the two techniques since the mean difference is of the order of + 0.19% oxygen saturation. The method studied is therefore sufficiently accurate; it does not need a special cell and does not require the determination of the total concentration of the haemoglobin.

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