蘿蔔S-locus glycoprotein基因型檢定及其序列比較

Abstract

In the experiment, the identification of SLG-alleles of radish was based on the PCRRFLP method; further the SLG-genes were sequenced and proteins were to deduced construct phylogenetic tree that predicted the incompatibilities of Salleles of radish. The genomic DNAs extracted from radish cvs. Rabano and Feng-fu No.2 were used as the template for PCR reaction. The amplified DNA fragments having length about 1160bp were digested by 12 restriction enzymes, these fragments were digested into 37 subfragments by Sal Ⅰ,StyⅠ、Hae Ⅲ XhoⅠ in the experiment the 12 SLGs were found based on he restriction enzyme patterns and gene sequences. The conserved N-glycosylation sites of the 12 SLG proteins deduced from gene sequences were predicted with the NetNGlyc 1.0 program, the results showed that the SLG3B has the highest number of 9 sites and the SLG2B only has the lowest of 4 sites. Gene and protein sequences of the 12 S-locus glycoproteins and other deposits of radish, cabbage and chinese cabbage in Gene-Bank, NCBI were pooled to construct the phylogenetic tree. The results showed that both cluster of SLG1A, SLG2A, SLG4A and SLG5B, SLG6A appeared the closest relationship of phylogenetic tree, the idex of compatibility of open flower pollination were 0 except SLG1A and SLG4A. Proteins both SLG2B and SLG3A were in same cluster they showed high idex of compatibility of open flower pollination. It was concluded that Salleles incompatibilities might be predicted based on the phylogenic tree of sequences of genes or proteins, it may be improved by scale up databank.