Abstract

(1) Background: Slightly acidic electrolyzed water (SAEW) is an effective and safe sterilizing solution. Its active component is hypochlorous acid (HOCl) which has been proved to exhibit a strong disinfectant activity. In this research we evaluated the effectiveness of SAEW in the removal of Methylobacterium mesophilicum, Rhodotorula mucilaginosa and Cladosporium cladosporioides, responsible for pink-colored biofilm and black mold in households. (2) Methods: Two concentrations of SAEW, 20 mg/L and 40 mg/L, were tested against M. mesophilicum, R. mucilaginosa and C. cladosporioides. In vitro experiments and mesh experiments were conducted to test the effectiveness of SAEW. (3) Results: The test results showed that 40 mg/L SAEW was effective in removing R. mucilaginosa and C. cladosporioides, with the population decreasing by approximately two orders of magnitude. For M. mesophilicum, resistance towards SAEW was observed; to obtain a 1.3 order of magnitude decrease in bacterial population, washing 5 times with 40 mg/L SAEW was necessary. Mesh experiments showed that SAEW can remove black mold; (4) Conclusions: Overall results indicated that SAEW was particularly effective for R. mucilaginosa and C. cladosporioides species commonly found in Japanese households.

Highlights

  • In 2020, the world was dedicated to the control and treatment of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic

  • Existing disinfectants were re-evaluated in each country, including hypochlorous acid water, which is known for its effectiveness, safety, and easy availability [1]

  • Results obtained in this study demonstrated that slightly acidic electrolyzed water (SAEW) effectively reduced colonies of Methylobacterium mesophilicum, Rhodotorula mucilaginosa and Cladosporium cladosporioides

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Summary

Introduction

In 2020, the world was dedicated to the control and treatment of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. Existing disinfectants were re-evaluated in each country, including hypochlorous acid water, which is known for its effectiveness, safety, and easy availability [1]. Hypochlorous acid is generally used for sanitation purposes in industries such as food production in concentrations ranging between 50 and. Water containing an extremely low concentration of HOCl can be considered an effective sterilizing agent while guaranteeing safety. Under the hypochlorous acid water umbrella, there are a great number of products that differ in manufacturing method and stability. As such, they should not be considered as a single product [4]. We paid attention to a type called slightly acidic electrolyzed water (SAEW)

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