Abstract
The present study aimed to investigate the influence of siRNA interference with a proliferation- inducing ligand (APRIL) gene on gastric carcinoma sgr-7901 cell apoptosis. Correlations between APRIL silencing and tyrosine kinase (trka) expression were also explored. Two APRIL-silencing siRNA vectors were constructed, and transfected into human gastric carcinoma sgr-7901 cells, expression before and after transfection being detected using RT-PCR and western blot analyses. The expression of 15 trka genes was detected using RT- PCR and apoptotic rates of sgr-7901 were assessed by flow cytometry. The expression levels of receptor trka genes were significantly decreased, and the apoptotic rate of sgr-7901 was significantly increased after transfection (P < 0.05). APRIL gene silencing can increase the apoptotic rate of gastric carcinoma cells, and inhibit the expression of receptor trka genes. There is a correlation between the signaling pathways of APRIL and trka.
Highlights
Gastric carcinoma is one of the most common tumors in the world
Researches in the past few years have shown that a proliferationinducing ligand (APRIL) is overexpressed in those patients with colon carcinoma who are resistant to 5-FU and have a low survival rate
It is proposed that APRIL is a new type of anti-5-FU gene, and can serve as a biological marker in predicting the survival rate of patients with colon carcinoma (Russell et al, 2009)
Summary
Gastric carcinoma is one of the most common tumors in the world. its incidence rate has decreasein recent years, it is still a type of malignant cancer with a high death rate, especially in East Asian countries including Korea, Japan and China (Stadtlander and Waterbor 1999; Wu et al, 2002; Hohenberger and Gretschel 2003; Jemal et al, 2006). Surgical treatment is a commonly-adopted method for gastric carcinoma patients, and has a good effect for patients at an early stage. Transcription inhibition through short-stranded oligonucleotides (like antisense DNA and RNA) is a newly-emerging molecule-targeting treatment method (Cho-Chung et al, 1999; 2003; Stephens and Rivers, 2003) It can inhibit the expression of such genes as bcl-2 (Konturek et al, 2001), bcl-XL (Lei et al, 2005), HER2 (Bao et al, 2010) and APRIL (Russell et al, 2009). Researches in the past few years have shown that APRIL is overexpressed in those patients with colon carcinoma who are resistant to 5-FU and have a low survival rate Base on this finding, it is proposed that APRIL is a new type of anti-5-FU gene, and can serve as a biological marker in predicting the survival rate of patients with colon carcinoma (Russell et al, 2009). The correlation between APRIL and receptor tyrosine kinase (trka) expression is explored
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