Abstract
Abstract DNA amplification reactions using long, non‐random, single oligonucleotide primers with thermostable DNA polymerase and avian genomic DNA generate unique, reproducible multiband patterns of DNA fragments. These DNA fragments are produced from amplification reactions using single primers of at least twenty‐five bases in length which were derived from the chicken αA‐globin gene. The patterns of amplified DNA fragments were polymorphic among individual chickens. Within families, polymorphic band patterns were found to be heritable, as the DNA fragments of progeny were present in at least one of the parents. In a conventional polymerase chain reaction containing a pair of primers, the relative concentrations of the primers can be manipulated to produce either a discrete two primer product or a multiband pattern identical to the single primer products. In addition, the number of bands in the multiband pattern increases as the stringency of the annealing step of the DNA amplification is decreased. The ...
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