Abstract
The interleukin 18 receptor 1 ( IL18R1 ) gene encodes a potent cytokine receptor that is critical for IL18 binding and subsequent signal transduction. This gene is a member of the IL1 receptor family that resides in a cluster of genes on human chromosome 2. Several polymorphisms have been shown to exist on this gene locus and some were found to be associated with inflammatory diseases but there is paucity of data on association with malaria. This study therefore, was aimed at determining the possible association of the rs1035130C>T coding polymorphism with severe malaria in Lafia, North-central Nigeria. The rs1035130C>T polymorphism was genotyped in a total of 214 participants including 98 severe malaria cases and 116 asymptomatic controls. DNA was extracted from blood spotted on filter paper using the QIAamp® DNA Mini Kit. The ABI PRISM® 3100 Genetic Analyzer was used to sequence the polymorphic locus. Our data showed a significantly higher frequency (P=0.021) of the CT heterozygous genotype in the asymptomatic control group (25.0%) than in the severe malaria group (13.27%). In addition, the frequency of the minor T allele was significantly higher (P=0.013) in the asymptomatic controls compared to the severe malaria cases. However, the TT homozygous genotype was not found in the severe malaria group. These results suggest a contributory role of the rs1035130C>T polymorphism in regulating host susceptibility or resistance to severe malaria and consequently have implications for understanding the molecular mechanisms of malaria pathogenesis. Keywords : Malaria, Interleukin, rs1035130C>T, Polymorphism, Genotype.
Highlights
Malaria continues to be a major global health problem with devastating impact on the health and livelihood of those affected
Genotyping was successfully performed for the 214 children enrolled into the study which includes 98 severe malaria cases and 116 asymptomatic controls
There was no deviation from the HardyWeinberg equilibrium (HWE) in the distributions of the rs1035130C>T genotypes in either the severe malaria group or the asymptomatic control group
Summary
Malaria continues to be a major global health problem with devastating impact on the health and livelihood of those affected. Even though progress has been achieved in recent times, following the widescale deployment of malaria control interventions (Bhatt et al, 2015), an estimated 212 million cases of malaria and 429 000 deaths were reported to have occurred globally in 2015 (WHO, 2016). Most of these cases were in sub-Saharan Africa; and Nigeria is the largest single country that is most affected, accounting for about 29% of the total malaria cases was reported to have occurred in 2015 (WHO, 2016). Biotech. 33 (2017): 113-119 malaria-related deaths and is common in subSaharan Africa (Murray et al, 2012; WHO, 2016)
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