Abstract
Electron micrography (EM) is an important method for determining the three-dimensional (3D) structure of macromolecular complexes and biological specimens. But there are several limitations such as poor signal-to-noise, limitation on range of tilt angles and sub-region subject to electron exposure, unintentional movements of the specimen, with EM systems that make the reconstruction procedure a severely ill-posed problem. A different choice of reconstruction method may lead to different results and create different additional artifacts in reconstructed images. In this paper, we combined the artifacts reduction strategy and the iterative reconstruction algorithm using a Mumford-Shah model. With the combined method, one can not only regularize the ill-posedness and provide segmentation simultaneously but also smooth additional artifacts due to the limited data. We applied the method to both simulated data from the Shepp-Logan phantom and cryo-specimen tomography. The results demonstrate the performance of the method in reducing the noise and artifacts while preserving and enhancing the edges in the reconstructed image.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.