Abstract
To evaluate the nutritional and functional value of Sipunculus nudus, a rapid, simple and sensitive analytical method was developed using ultra-high performance liquid chromatography coupled with a triple quadrupole mass detection in multiple-reaction monitoring mode for the simultaneous quantitative determination of 25 free amino acids and 16 nucleosides and nucleobases in S. nudus within 20 min, which was confirmed to be reproducible and accurate. The limits of detection (LODs) and quantification (LOQs) were between 0.003–0.229 μg/mL and 0.008–0.763 μg/mL for the 41 analytes, respectively. The established method was applied to analyze 19 batches of S. nudus samples from four habitats with two different processing methods. The results showed that S. nudus contained a variety of free amino acids, nucleosides and nucleobases in sufficient quantity and reasonable proportion. They also demonstrated that the contents of these compounds in different parts of S. nudus were significantly discriminating, which were in the order: (highest) coelomic fluid > body wall > intestine (lowest). The method is simple and accurate, and could serve as a technical support for establishing quality control of S. nudus and other functional seafoods. Moreover, the research results also laid foundation for further exploitation and development of S. nudus.
Highlights
Sipunculus nudus, a Sipunculid species, known as sandworm or haichangzi, has a practically global distribution, except for polar waters
To evaluate the free amino acids, nucleosides and nucleobases of S. nudus extracted in the traditional way, the extraction method of refluxing for 60 min was applied to imitate water decoction
The limit of detection (LOD) and limit of quantification (LOQ) ranged from 0.003–0.229 μg/mL and 0.008–0.763 μg/mL, respectively
Summary
Sipunculus nudus, a Sipunculid species, known as sandworm or haichangzi, has a practically global distribution, except for polar waters. They are unsegmented wormlike animals, comprising two sections namely the trunk (main body) and an introvert (extending and contracting neck-like “feeler”) [1]. S. nudus extract was reported to be rich in a variety of nutritional and functional components consisting of free amino acids, fatty acids, polysaccharides, mineral elements and so on. In order to compile comprehensive information about the nutritional and functional components in S. nudus, we performed a preliminary experiment to detect the nucleosides and nucleobases in S. nudus and found such constituents were abundant in S. nudus water extract
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