Abstract
Furfurals, including furfural (F), 5-hydroxymethylfurfural (5-HMF), 2-acetylfuran (FMC), and 5-methyl-2-furfural (MF), are important Maillard reaction intermediate products in dairy products. Due to the complex matrix of cheese samples and different polarity of four furfurals, it remains a major challenge to simultaneously determine these four furfurals in cheeses. A new method for simultaneous determination of four furfurals in cheeses by gas chromatography-triple quadrupole mass spectrometry (GC-MS/MS) was established in the present study. The limits of detection (LODs) were in the range of 0.01 - 0.02 mg/kg, and the correlation coefficient (R2) for each analyte was above 0.9986 with the calibration range established from 5 to 1000 ng/mL. The recoveries were ranged from 72.62% to 123.29% and relative standard deviations (RSDs) lower than 9.82%. Finally, the developed method was successfully employed for analyzing four furfurals in 30 cheese samples, and trace levels of the furfurals were found in cheeses.
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