Abstract
The objective of this study was to determine the possibility of simultaneous biosynthesis of lipids and carotenoids by the Rhodotorula yeast strains in media with waste glycerol and deproteinized potato wastewater and to determine the level of pollution reduction by media. On the basis of results obtained during the yeast microcultures in the Bioscreen C system, it was found that potato wastewater and glycerol can be used as components of media for Rhodotorula glutinis, Rhodotorula mucilaginosa, and Rhodotorula gracilis yeast strains. The amount of glycerol added to media higher than 10% significantly decreased the growth rate of yeast. The results of yeast culture in the laboratory shaker flasks showed a possibility of simultaneous production of lipids and carotenoids by R. glutinis, R. mucilaginosa, and R. gracilis yeast strains during cultivation in media containing only waste glycerol and deproteinized potato wastewater. A higher intracellular lipid content (approximately 15 g/100 gd.w.) was obtained for R. mucilaginosa and R. gracilis yeast biomass after cultivation in experimental media with waste glycerol and potato wastewater. In conclusion, the yeast grown in media with potato wastewater supplemented with 3% or 5% glycerol synthesized carotenoids, and their content in biomass did not exceed 230 μg/gd.w.
Highlights
The ability of yeast to synthesize large amounts of lipids became known to scientists at the beginning of the twentieth century
Rhodotorula glutinis LOCKR13 was obtained from the Collection of Pure Cultures of the Lodz University of Technology (Poland), while the strains R. mucilaginosa ATCC 66034 and R. gracilis ATCC 10788 were obtained from the American Collection of Pure Cultures (USA)
The study showed that it is possible to simultaneously biosynthesize lipids and carotenoids by R. glutinis, R. mucilaginosa, and R. gracilis yeast when growing in a medium containing only waste glycerol and deproteinized potato wastewater (PW)
Summary
The ability of yeast to synthesize large amounts of lipids became known to scientists at the beginning of the twentieth century. Intensive research is being conducted worldwide on the possibility of producing microbial-derived lipids on an industrial scale. This stems primarily from the fact that this process is independent of climatic conditions, requires less manual intervention, has a short production cycle, and enables the fatty acid composition to be modified by regulating culture parameters. Yeasts are excellent producers of microbial lipids because of their high content in cellular biomass. These microorganisms exhibit a rapid growth rate and low nutritional requirements, and the fatty acid composition can be modified by changing the culture conditions [2]. The group of oleaginous yeasts includes yeasts of genus Rhodotorula, which apart from lipids can synthesize other valuable cellular components such as carotenoids [3]
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