Abstract
A simple, precise, rapid, selective, and economic reversed phase high-performance liquid chromatography (RP-HPLC) method has been established for simultaneous analysis of A Phenomenex C18 (250×4.6 mm i.d) chromatographic column equilibrated with mobile phase 0.02 M Potassium dihydrogen o-phosphate/acetonitrile (55/45, v/v) adjusted to pH 6.5 with Triehtylamine (1% v/v) was used. Mobile phase flow rate was maintained at 1 ml/min and effluents were monitored at 278 nm. The sample was injected using a 20 μl fixed loop, and the total run time was 10 min. Experimental conditions such as pH of mobile phase, column saturation time, selection of wavelength, etc. were critically studied and the optimum conditions were selected. The retention time for PCM, DMP and TMD were 3.76 min, 5.18 min and 4.28 min, respectively. The calibration curve for DMP, PCM and TMD was found to be linear in the range of 0.2–1 μg/ml, 6.5–32.5 μg/ml and 0.75–3.75 μg/ml with a correlation coefficient of 0.9998, 0.9976 and 0.9974. The detection limits for PCM, DMP and TMD were 20 ng/ml, 1.06 ng/ml and 2 ng/ml, respectively, while quantitation limits were 60 ng/ml, 3.23 ng/ml and 6 ng/ml, respectively. This HPLC procedure is economic, sensitive, and less time consuming than other chromatographic procedures. It is a user-friendly and importance tool for analysis of combined tablet dosage forms.
Highlights
PCM (PCM; N-[4-hydroxyphenyl] ethanamide; Figure 1) is a widely used analgesic and antipyretic for the relief of fever, headaches, and other minor aches and pains, and is a major ingredient in numerous cold and flu remedies
DMP is official in Indian Pharmacopoeia where assay is described by titrimetric method
Literature survey revealed that various analytical methods like spectrophotometric [3,4,5,6], HPLC [7,8,9,10,11,12,13,14], GC [15] and HPTLC [16,17,18,19] have been reported for the determination of TMD, PCM and either individually or combination with some other drugs, but no HPTLC method was reported for HN N O
Summary
PCM (PCM; N-[4-hydroxyphenyl] ethanamide; Figure 1) is a widely used analgesic and antipyretic for the relief of fever, headaches, and other minor aches and pains, and is a major ingredient in numerous cold and flu remedies. This pharmacopoeia suggests titrimetric and UV spectrophotometric assay method for PCM in bulk and tablet formulations. DMP is official in Indian Pharmacopoeia where assay is described by titrimetric method. This pharmacopoeia suggests titrimetric (potentiometric) assay method for tramadol in bulk. Literature survey revealed that various analytical methods like spectrophotometric [3,4,5,6], HPLC [7,8,9,10,11,12,13,14], GC [15] and HPTLC [16,17,18,19] have been reported for the determination of TMD, PCM and either individually or combination with some other drugs, but no HPTLC method was reported for HN N O
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