Abstract
Objective To establish a method for the simultaneous content determination of quercetin, kaempferol and pinocembrin in Penthorum Chinense Pursh. Methods The HPLC analysis was carried out on Alltima C18 (250 mm × 4.6 mm, 5 μm) with a mixture of methanol-0.2% phosphate (45:55) as the mobile phase. The determination wavelength was set at 270 nm with the flow rate of 1.0 ml/min. The column temperature was set at 30 ℃. Results The quercetin , kaempferol and pinocembrin showed good linearity in the range of 0.316-10.120 μg (r=0.999 9), 0.012-0.397 μg (r=0.999 6) and 0.063-2.016 μg (r=0.999 8) respectively. The average recovery rates of quercetin, kaempferol and pinocembrin were 99.34% (RSD=1.51%), 99.76% (RSD=1.96%) and 98.34% (RSD=1.56%) respectively. Conclusions The method is accurate and sensitive, which can be used for the content determination of quercetin, kaempferol and pinocembrin in Penthorum Chinense Pursh. Key words: Penthorum Chinense pursh; Quercetin; Kaempferol; Pinocembrin; High performance liquid chromatography
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