Abstract
A vortex-assisted dispersive liquid-liquid microextraction was proposed based on hydrophobic deep eutectic solvent and high performance liquid chromatography for the quantitative study of five small molecular compounds (tangshenoside I, lobetyolinin, lobetyolin, lobetyol, atractylenolide III) and quality evaluation of Codonopsis Radix. In this experiment, the primary factors impacting the extraction competence process were optimized. Under optimal conditions that were DES (methyltrioctylammonium chloride: glycerol, molar ratio 1:4, 70 μL), pH = 7, vortex time of 90 s, centrifugal time of 2 min, enrichment factors of 6.0, 6.2, 18.9, 58.7, 63.2 for tangshenoside I, lobetyolinin, lobetyolin, lobetyol, atractylenolide III were obtained. The detection limits of the above five components were respectively 9, 6, 2, 0.1, 0.6 µg/L and five analytes had excellent linearities in individual linear ranges (R2 > 0.990). Good precision and recovery were 0.5%-8.8% and 92.3%-111.5%. The quantitative results revealed that the proposed approach is quick, simple, feasible, and could accurately detect the contents of five target analytes in 18 batches samples. Finally, five components could be chemical markers to objectively distinguish different specifications of Codonopsis Radix by analysis of variance, further contributing a foundation for quality evaluation and control of Codonopsis Radix.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
