Abstract
Coffee is a favorite and beverage in Western countries that is consumed daily. In the present study, capillary zone electrophoresis (CE) was applied for the separation and quantification of three isoflavones including daidzein, genistein and formononetin in coffee. Extraction of isoflavones from the coffee sample was carried out by extraction and purification process using ether after the acid hydrolysis with the antioxidant butylated hydroxy-toluene (BHT). The experimental conditions of the CE separation method were: 20 mmol/L Na2HPO4 buffer solution, 25 kV applied voltage, 3 s hydrodynamic injection at 30 mbar, and UV detection at 254 nm. The results show that the three compounds can be tested within 10 min with a linearity of 0.5–50 µg/mL for all three compounds. The limits of detection were 0.0642, 0.134, and 0.0825 µg/mL for daidzein, formononetin and genistein, respectively. The corresponding average recovery was 99.39% (Relative Standard Detection (RSD) = 1.76%), 98.71% (RSD = 2.11%) and 97.37% (RSD = 3.74%).
Highlights
Phytoestrogens (PEs), called “dietary estrogens”, are a group of natural, non-steroidal, estrogen-like compounds found in a wide variety of plants consumed by humans [1]
The proposed method was successfully applied to using ether instead of ethanol after the process of acid hydrolysis with the antioxidant butylated the determination of three isoflavones in commercially available coffee samples
It was necessary to determine the parameters of capillary zone electrophoresis (CE) before analyzing the daidzein, genistein and formononetin in the coffee sample
Summary
Phytoestrogens (PEs), called “dietary estrogens”, are a group of natural, non-steroidal, estrogen-like compounds found in a wide variety of plants consumed by humans [1]. Addition, Fromisthe works reported so far, one canwith see that quantitative determination of In isoflavones the separation system is acidic. We improved the purification process ether from instead ethanol after thean process of acid the extraction method of using isoflavones theof coffee sample with extraction and hydrolysis purificationwith process antioxidant butylated hydroxy-toluene (BHT). The proposed method was successfully applied to using ether instead of ethanol after the process of acid hydrolysis with the antioxidant butylated the determination of three isoflavones in commercially available coffee samples. The sample the comparative cost is isrelatively lower It can used as for the preparation of this method simple, the analytical timebe is shorter, andan thealternative comparativemethod cost is relatively determination of isoflavones in coffee.
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