Abstract
The pharmacodynamic effect of longan leaves was attributed to various components, especially the flavonoids. In this paper, a new strategy of quantitative analysis of multicomponents by a single marker (QAMS) method was first established to synchronously determine 5 components (ethyl gallate (C1), astragalin (C2), quercetin (C3), luteolin (C4), and kaempferol (C5)) in Dimocarpus longan by ultra-performance liquid chromatography (UPLC) and high-performance liquid chromatography (HPLC). Quercetin (C3) was chosen as the internal reference. Relative correction factors (RCFs, ƒs/i) of the other 4 components were calculated by two correction methods (multipoint correction and slope correction) to effectuate QAMS. At the same time, the difference between the results measured by the QAMS and external standard methods was compared to verify the accuracy of QAMS. Within the linear range, the results showed that all ƒs/i values were obtained with good durability under diverse chromatographic conditions (RSD < 2.28%). The quantitative results of 5 components in the leaves of Dimocarpus longan collected from 10 producing areas by different chromatographic systems and quantitative methods were significantly correlated (Pearson's r > 97.0%). The applicability and feasibility of the QAMS method established in this study were evaluated to be favorable for quality control of the leaves of Dimocarpus longan. As a new model of quality control, it can provide one more choice of multicomponent quality-control method in the absence of standard substances or instruments.
Highlights
Quantitative analysis of multicomponents by a single marker (QAMS) is a new mode of multi-index evaluation
Quercetin (C3) as an internal reference for its stable property, low price, and acquiring standard substance was used to calculate RCFs of the other 4 components. e QAMS method was first established to control the quality of Dimocarpus longan leaves more conveniently, comprehensively, and synthetically, investigating the feasibility of ultraperformance liquid chromatography (UPLC) and high-performance liquid chromatography (HPLC) methods based on chromatographic condition transformation
Representative HPLC/UPLC Chromatograms. e chromatograms of mixed standard solutions and sample solutions were taken for analysis under the aforementioned corresponding conditions. e results testified that separation of the 5 components in Dimocarpus longan leaves with the peaks freed from interference by adjacent peaks was good
Summary
Quantitative analysis of multicomponents by a single marker (QAMS) is a new mode of multi-index evaluation. The QAMS method has been used successfully in the quality control of various natural plant medicines in many countries [4]. It has been collected in the China Pharmacopoeia (the 2015 Edition) to evaluate the quality of Coptis chinensis [5]. A new strategy of quantitative analysis of multicomponents by a single marker (QAMS) method for simultaneous quantification of 5 components in Dimocarpus longan by UPLC and HPLC was developed. E QAMS method was first established to control the quality of Dimocarpus longan leaves more conveniently, comprehensively, and synthetically, investigating the feasibility of UPLC and HPLC methods based on chromatographic condition transformation Quercetin (C3) as an internal reference for its stable property, low price, and acquiring standard substance was used to calculate RCFs of the other 4 components. e QAMS method was first established to control the quality of Dimocarpus longan leaves more conveniently, comprehensively, and synthetically, investigating the feasibility of UPLC and HPLC methods based on chromatographic condition transformation
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