Simultaneous detection of airborne Aflatoxin, Ochratoxin and Zearalenone in a poultry house by immunoaffinity clean-up and high-performance liquid chromatography

Abstract

An AOZ method, based on high-performance liquid chromatography (HPLC), was optimized on HPLC condition such as mobile phase and wavelength to simultaneously quantify six kinds of mycotoxins [four aflatoxins (AFs), ochratoxin A (OTA) and zearalenone (ZEA)]. Conditions for immunoaffinity clean-up, HPLC and photo-derivatization were optimized in this study and successfully applied in assessment of airborne mycotoxins from a poultry house in Dalian, China. Fifty-two air samples were collected with AGI-30 air samplers using pure water as collection media. Twenty air samples (20/52, 38.46%) were positive for four toxins. Among the positive samples, airborne mycotoxin concentrations (mean±S.D.) for AFG 2, AFB 1, and ZEA were 0.189±0.024 ( n=9), 0.080±0.003 ( n=11) and 2.363±0.030 ( n=5) ng/m 3 air, while the concentration for OTA was 8.530 ( n=1) ng/m 3. No positive sample was found for either AFG 1 or AFB 2. A chicken may inhale 0.019–0.057 ng AFG 2, 0.013–0.019 ng AFB 1, 0.436–0.513 ng ZEA, and 1.706 ng OTA, respectively, in a day. A poultry worker may inhale 0.504–1.512 ng AFB 1, 0.752–2.28 ng AFG 2, 68.240 ng OTA, and 17.432–20.512 ng ZEA in a working day. This is the first report on airborne mycotoxins in poultry house. These data may have importance in animal and public health implications.