Abstract
We have developed a new Superagglutination test for serodiagnosis of infectious diseases. It differs from conventional plate/slide agglutination tests (PAT/SAT) by three additional steps: prior staining of serum antibody by adding a dye and addition of diluted biotinylated antiglobulin and avidin in sequence after mixing the antigen with the test serum. The new steps circumvent the problems of false positive and false negative results of PAT/SAT. In serodiagnosis of brucellosis, Superagglutination test had higher positive predictive value and specificity than Rose Bengal Plate Test (RBPT) and Standard Tube Agglutination Test (STAT) and higher negative predictive value and sensitivity than RBPT, STAT, ELISA and Complement Fixation Test (CFT).•Superagglutination is a simple, accurate and economic screening test for infections.•More specificity, sensitivity, positive & negative predictive value than RBPT, STAT.•More sensitivity, negative predictive value than ELISA and Complement Fixation Test.
Highlights
In many countries, the standard Plate Agglutination Test is the routine screening test for human and animal brucellosis
Rose Bengal Plate Test (RBPT) is a variant of plate/slide agglutination test where killed Brucella organisms stained with Rose Bengal dye are used as antigen for detection of antibodies in the serum
Brucellosis suspected herds were selected for sampling primarily based on the history of abortions in the herd while normal healthy animals were sampled from the herds of the university dairy farm without the history of abortions and with repeatedly Rose Bengal Plate Test (RBPT) negative status
Summary
The standard Plate Agglutination Test is the routine screening test for human and animal brucellosis. The RBPT is a quick, cheap and effective test for the diagnosis of brucellosis It may give false negative results [1,2]. False negative results may be due to a small clump size in sera with low titers of antibodies. Prozoning may often lead to a false negative reaction in RBPT when sera of high antibody titers are tested against it. It has been suggested [5,6] that in order to get a better diagnosis of Brucella infection, a combination of RBPT and ELISA should be used, especially in case of samples found negative by either RBPT or STAT used alone or in combination
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