Abstract
Oxytetracycline (OTC) is a common antibacterial agent used for the control of animal diseases. OTC abuse can seriously affect human health. Herein, based on the Fe(III)-3,3’,5,5’-tetramethylbenzidine (Fe(III)-TMB) system, a facile and rapid colorimetricassay for oxytetracycline (OTC) was successfully developed. The addition of OTC could remarkably enhance the Fe(III)-oxidized TMB reaction and the absorbance increase of Fe(III)-TMB solution is proportional to the added OTC. The linear range of proposed sensor for OTC was from 20 nM to 1000 nM with the detection limit of 7.97 nM. The high sensitivity for OTC detection was successfully achieved under optimal conditions. For real sample analysis, recoveries of 89.93% to 100.02% was obtained. This is the first report for detecting OTC based on the nonenzymatic colorimetric reaction using the intrinsic oxidized activity of OTC/Fe3+ complex. The present simple, low-cost and visualized sensor has great potential for OTC detection in food.
Highlights
Oxytetracycline (OTC) is a member of the most common broad-spectrum tetracycline (TCs) group of antibiotics
The linear range of proposed sensor for OTC was from 20 nM to 1000 nM with the detection limit of 7.97 nM
To demonstrate the feasibility of our approach, the TMB oxidation was first studied in different systems (Figure 1(a))
Summary
Oxytetracycline (OTC) is a member of the most common broad-spectrum tetracycline (TCs) group of antibiotics It has been widely used as veterinary drug and feed additive in livestock production for the treatment of infectious diseases and promote growth, due to its effective antimicrobial properties and low cost [1]. Various analytical methods have been proposed for the detection of OTC, such as chromatography methods [7] [8], fluorescence method [9] [10], and electrochemistry [11] [12], etc Though most of these methods have high sensitivity, they suffer from the disadvantages of high costs, time-consuming, complicated sample pretreatment and sophisticated instrument manipulation, which limit their application for rapid, on-site and real-time determination. The present approach has been successfully applied to determine OTC in honey samples
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
